pinp elisa kit  (Cusabio)

Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: The differences of optimal vibration frequency for accelerating OTM between the younger group and the older group (A and B) Micro-CT images and statistical analysis showed the orthodontic teeth of the younger rats in L-Vib group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (C) The content of CTX-1 in serum of the younger rats in control group, L-Vib group and H-Vib group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. (D and E) Representative images of TRAP staining of alveolar bone of the younger rats in control group, L-Vib group and H-Vib group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (F) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the younger rats in control group, L-Vib group and H-Vib group. Scale bar: 100 μm. (G) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in L-Vib group were higher than those in control group and H-Vib group. Mean ± SD. n = 3. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (H and I) Micro-CT images and statistical analysis showed the orthodontic teeth of the older rats in H-Vib group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5. ∗ p < 0.05. ∗∗∗ p < 0.001. (J) The content of CTX-1 in serum of the older rats in control group, L-Vib group and H-Vib group was detected by ELISA. Mean ± SD. n = 3–4. ∗ p < 0.05. ∗∗ p < 0.01. (K and L) Representative images of TRAP staining of alveolar bone of the older rats in control group, L-Vib group and H-Vib group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗∗ p < 0.001. (M) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the older rats in control group, L-Vib group and H-Vib group. Scale bar: 100 μm. (N) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in H-Vib group were higher than those in control group and L-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. Statistical significance was determined using the one-way ANOVA.

Article Snippet: PINP ELISA kit , CUSABIO , CSB-E12774r.

Techniques: Micro-CT, Control, Enzyme-linked Immunosorbent Assay, Staining, Expressing

Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: The synergistic effect of vibration and NIR light on OTM (A) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the younger rats in control group, L-Vib group and synergy group. Scale bar: 100 μm. (B) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in synergy group were higher than those in control group and L-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (C and D) Representative images of TRAP staining of alveolar bone of the younger rats in control group, L-Vib group and synergy group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4. ∗ p < 0.05. ∗∗∗ p < 0.001. (E) The content of CTX-1 in serum of the younger rats in control group, L-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. (F and G) Micro-CT images and statistical analysis showed the orthodontic teeth of the younger rats in synergy group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5–6. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (H) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the older rats in control group, H-Vib group and synergy group. Scale bar: 100 μm. (I) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in synergy group were higher than those in control group and H-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (J and K) Representative images of TRAP staining of alveolar bone of the older rats in control group, H-Vib group and synergy group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (L) The content of CTX-1 in serum of the older rats in control group, H-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗∗ p < 0.001. (M and N) Micro-CT images and statistical analysis showed the orthodontic teeth of the older rats in synergy group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5–6. ∗∗∗ p < 0.001. Statistical significance was determined using the one-way ANOVA.

Article Snippet: PINP ELISA kit , CUSABIO , CSB-E12774r.

Techniques: Control, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Micro-CT

Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: Light-vibration coordination accelerates OTM without root resorption and alveolar bone resorption (A and B) Micro-CT images and statistical analysis showed that the moving distance of orthodontic tooth was increased in synergy group of the younger rats after orthodontic force application for 4 weeks. Scale bar: 2 mm. Mean ± SD. n = 5. ∗∗ p < 0.01. (C) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in synergy group were higher than those in control group after orthodontic force application for 4 weeks. Mean ± SD. n = 3. ∗∗∗ p < 0.001. (D and E) Micro-CT images and statistical analysis showed that the moving distance of orthodontic tooth was increased in synergy group of the older rats after orthodontic force application for 4 weeks. Scale bar: 2 mm. Mean ± SD. n = 6. ∗∗ p < 0.01. (F) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in synergy group were higher than those in control group after orthodontic force application for 4 weeks. Mean ± SD. n = 3. ∗∗ p < 0.01. (G) Representative images of HE staining of the longitudinal sections of the maxillary first molar of the younger rats. Scale bar: 500 μm. (H and I) There was no significant difference in the level of root resorption and alveolar bone resorption between the control group and the synergy group of the younger rats. Mean ± SD. n = 4. ns, not significant. (J) The content of PINP in serum of the younger rats in control group, L-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3–4. ∗∗∗ p < 0.001. (K) WB results showed that the protein expression levels of RUNX2 and OSX of the younger rats in synergy group were higher than those in control group. Mean ± SD. n = 3. ∗∗ p < 0.01. (L) Representative images of HE staining of the longitudinal sections of the maxillary first molar of the older rats. Scale bar: 500 μm. (M and N) There was no significant difference in the level of root resorption and alveolar bone resorption between the control group and the synergy group of the older rats. Mean ± SD. n = 3–4. ns, not significant. (O) The content of PINP in serum of the older rats in control group, H-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3–4. ∗∗∗ p < 0.001. (P) WB results showed that the protein expression levels of RUNX2 and OSX of the older rats in synergy group were higher than those in control group. Mean ± SD. n = 3. ∗∗ p < 0.01. Statistical significance was determined using the Student’s t tests one-way ANOVA.

Article Snippet: PINP ELISA kit , CUSABIO , CSB-E12774r.

Techniques: Micro-CT, Expressing, Control, Staining, Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: RKIP regulates bone marrow macrophage differentiation to mediate osteoclastogenesis and H-type vessel formation

doi: 10.1038/s41467-025-62972-8

Figure Lengend Snippet: A Micro-CT and three-dimensional (3D) reconstruction images of the distal femur of 3-month-old male RKIP +/+ or RKIP -/- mice are shown. B Quantification of bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular separation (Tb.Sp), trabecular thickness (Tb.Th), and cortical thickness (Ct.Th) ( n = 5). C H&E staining and TRAP staining of 3-month-old male RKIP +/+ or RKIP -/- mice femurs sections. D Quantification of osteoclast number per bone surface (N.Oc/BS) and osteoclast surface per bone surface (Oc.S/BS) ( n = 6). E Calcein double staining and Von Kossa staining of 3-month-old male RKIP +/+ or RKIP -/- mice femurs sections. F Quantification of mineral apposition rate (MAR) and bone formation rate per bone surface (BFR/BS) ( n = 6). G OCN (green) and DAPI (blue) immunofluorescence of 3-month-old male RKIP +/+ or RKIP -/- mice femurs sections; The arrow indicates the cells which express OCN. H Quantification of OCN + cell number per bone perimeter (N.OCN + /B.Pm) ( n = 6). I Serum C-terminal telopeptide of type I collagen (CTX-1) and Procollagen I N-Terminal Propeptide (PINP) concentration measured by ELISA from RKIP +/+ or RKIP -/- male mice ( n = 6). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01 for a comparison with the control group or as indicated. n value means the number of repetitions in each independent experiment.

Article Snippet: Locostatin (#HY-W013411A, MCE, USA), Didymin (#HY-N2068, MCE, USA), recombinant mouse M-CSF (#CB34, Novoprotein, China) and RANKL (#462-TEC, R&D, USA), recombinant human M-CSF (#C417, Novoprotein, China) and RANKL (#CK63, Novoprotein, China), TRAP staining kits (#387A-1KT, Sigma-Aldrich, USA), Mouse CTX-1 ELISA Kit (#CSB-E12782m, CUSABIO, China), Mouse PINP ELISA Kit (#CSB-E12775m, CUSABIO, China) were used.

Techniques: Micro-CT, Staining, Double Staining, Immunofluorescence, Concentration Assay, Enzyme-linked Immunosorbent Assay, Comparison, Control

Journal: Bone Research

Article Title: Bone marrow adipogenic lineage precursors are the major regulator of bone resorption in adult mice

doi: 10.1038/s41413-025-00405-4

Figure Lengend Snippet: Depletion of RANKL in MALPs increases long bone trabecular bone mass in adult mice by suppressing bone resorption. a qRT-PCR analysis of Rankl mRNA in bone marrow and cortical bone from WT and RANKL iCKO mice at 4 weeks after Tam injection. Mice received Tam at 3 months of age. n = 3 mice/group. b ELISA analysis of RANKL in bone marrow from WT and RANKL iCKO mice at 2 weeks after Tam injection. Mice received Tam at 3 months of age. n = 3 mice/group. c 3D microCT reconstruction of whole femurs from WT and iCKO mice at 1 month after Tam injection. Scale bar = 1 mm. d 3D microCT reconstruction reveals a drastic increase of femoral trabecular bone. Scale bar = 200 µm. e MicroCT measurement of trabecular bone structural parameters. BV/TV bone volume fraction, Tb.N trabecular number, Tb.Th trabecular thickness, Tb.Sp trabecular separation. f Representative TRAP staining images show TRAP+ osteoclast (arrows) at different skeletal sites: secondary spongiosa (SS), chondro-osseous junction (COJ), and endosteal surface (Endo.S). Scale bar = 50 μm. g Quantification of osteoclast surface (Oc.S) at 3 skeletal sites. BS bone surface, L COJ length. h Representative Osterix staining of trabecular bone from WT and RANKL iCKO femurs. Scale bar = 50 μm. i Quantification of osteoblast surface (OB.S). j Representative double labeling of trabecular bone from WT and iCKO femurs. Scale bar = 20 μm. k Bone formation activity is quantified. MAR mineral apposition rate, MS mineralizing surface, BFR bone formation rate. l Serum ELISA analysis of bone resorption marker (CTX-1) and formation marker (P1NP) in WT and iCKO mice. * P < 0.05; ** P < 0.01; *** P < 0.001 vs WT , n = 5–6 mice/group

Article Snippet: Sera were collected during mouse euthanization for measuring bone turnover markers, collagen type I C-telopeptide degradation products (mouse CTX-I ELISA Kit, MyBioSource), N-terminal propeptide of type I procollagen (ImmunotagTM Mouse PINP ELISA Kit, G-Bioscience) according to the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Injection, Enzyme-linked Immunosorbent Assay, Staining, Labeling, Activity Assay, Marker

Journal: Bone Research

Article Title: Bone marrow adipogenic lineage precursors are the major regulator of bone resorption in adult mice

doi: 10.1038/s41413-025-00405-4

Figure Lengend Snippet: RANKL deficiency in MALPs protects adult female mice from ovariectomy-induced trabecular bone loss. a 3D microCT reconstruction of femoral trabecular bone from WT and RANKL iCKO mice at 6 weeks post OVX surgery. Mice received Tam injections at 3 months of age right before the surgery. Scale bar = 200 µm. b MicroCT measurement of trabecular bone structural parameters. c Representative TRAP staining images of trabecular bone from WT and RANKL iCKO femurs show TRAP + osteoclasts (arrows). Scale bar = 50 μm. d Quantification of osteoclast surface (Oc.S). e Representative Osterix staining of trabecular bone from WT and RANKL iCKO femurs. Scale bar = 50 μm. f Quantification of osteoblast surface (OB.S). g Bone formation activity is quantified. h Serum ELISA analysis of bone resorption marker (CTX-1) and formation marker (P1NP) in WT and iCKO mice. i Representative H&E staining of trabecular bone from WT and RANKL iCKO femurs. Scale bar = 50 μm. j Quantification of the percentage of adipocyte area within bone marrow and adipocyte size. # P < 0.05; ## P < 0.01; ### P < 0.001 OVX vs Sham; * P < 0.05; ** P < 0.01; *** P < 0.001 iCKO vs WT , n = 5–6 mice/group

Article Snippet: Sera were collected during mouse euthanization for measuring bone turnover markers, collagen type I C-telopeptide degradation products (mouse CTX-I ELISA Kit, MyBioSource), N-terminal propeptide of type I procollagen (ImmunotagTM Mouse PINP ELISA Kit, G-Bioscience) according to the manufacturer’s instructions.

Techniques: Staining, Activity Assay, Enzyme-linked Immunosorbent Assay, Marker

Journal: Bone Research

Article Title: Bone marrow adipogenic lineage precursors are the major regulator of bone resorption in adult mice

doi: 10.1038/s41413-025-00405-4

Figure Lengend Snippet: Depleting RANKL in MALPs in osteoporotic mice restores trabecular bone mass. a 3D microCT reconstruction of femoral trabecular bone from WT and RANKL iCKO mice at 10 weeks post OVX surgery. Mice received the surgery at 3 months of age and vehicle or Tam injections 6 weeks later. Scale bar = 200 µm. b MicroCT measurement of trabecular bone structural parameters. c Representative TRAP staining images of femoral trabecular bone from WT and RANKL iCKO mice with vehicle or Tam injections show TRAP + osteoclasts (arrows). Scale bar = 50 μm. d Quantification of osteoclast surface (Oc.S). e Representative Osterix staining of femoral trabecular bone from WT and RANKL iCKO mice with vehicle or Tam injections. Scale bar = 50 μm. f Quantification of osteoblast surface (OB.S). g Bone formation activity is quantified. h Serum ELISA analysis of bone resorption marker (CTX-1) and formation marker (P1NP) in WT and iCKO mice with vehicle or Tam injections. # P < 0.05; ## P < 0.01; ## P < 0.001 Tam vs Veh; * P < 0.05; ** P < 0.01; *** P < 0.001 iCKO vs WT , n = 5–6 mice/group

Article Snippet: Sera were collected during mouse euthanization for measuring bone turnover markers, collagen type I C-telopeptide degradation products (mouse CTX-I ELISA Kit, MyBioSource), N-terminal propeptide of type I procollagen (ImmunotagTM Mouse PINP ELISA Kit, G-Bioscience) according to the manufacturer’s instructions.

Techniques: Staining, Activity Assay, Enzyme-linked Immunosorbent Assay, Marker